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PусскийGood news! Not only a leading enterprise has announced the release of the first completely reusable 3D cell culture microplate on the market, but also another one is excited to introduce the new 96-well ultra low adherence cell culture plate specifically for culturing spheroids. This means both disposable and reuseable cell culture plates can be used in labs or hospitals.
Reusable 3D Cell Culture Microplate:
Scientific research presently generates significant amounts of single use plastics, ranging from serological pipettes and pipette tips to vials and culture microplates. These plastic items contribute to the daily waste in laboratories. However, the timing is opportune for the introduction of the completely reusable EB-Plate, especially considering the high demand for 3D cell culture microplates, the scarcity of conventional plastic plates, and the growing popularity of the zero-waste movement in laboratory settings.
The environmentally conscious EB-Plate, which is both reusable and nearly indestructible, enters the market, albeit at a higher cost compared to its plastic counterparts, aligning with the trend towards sustainability. The company claims that these new plates become cost-effective after approximately five to eight uses, and their durability allows for numerous additional reuses, making them a financially viable and eco-friendly choice over the long term.
While acknowledging the shift from traditional devices to innovative tools, the assertion regarding the EB-Plate emphasizes that scientists need not compromise on quality when transitioning from conventional single use microplates to a more sustainable option. This newly introduced plate facilitates the generation of embryoid bodies, serving as the crucial initial step in cultivating larger self-assembled 3D cultures, such as spheroids or organoids, from stem cells.
The distinctive microwell geometry of the EB-Plate ensures the consistent creation of uniformly-sized spheroids with high reproducibility. Additionally, the plate's high-transmissive bottom enhances optical observation quality. The company foresees enduring scientific and economic benefits for researchers, as this environmentally-friendly device proves to be a valuable asset in the long run.
96-Well Ultra Low Adherence Cell Culture Plate
The surface of the 96-well ultra low adherence cell culture plate is designed to be anti adhesive, effectively repelling cells of all types and preventing them from adhering to the plate. Combined with the round bottoms of the wells, this feature promotes the growth of cells into 3D spheroids. The specialized surface of the 96 well cell strainer facilitates rapid spheroid formation, occurring within 8 to 24 hours, exhibiting exceptional circularity and reproducibility compared to alternative surfaces.
The robust chemical composition of the 96-well cell culture plate surface imparts high durability, allowing it to withstand multiple washing steps and mechanical actions, such as pipetting. Moreover, this ultra low adherence surface has demonstrated remarkable reliability, even with challenging cell types. Numerous cell lines, including some primary cells, have undergone successful testing for spheroid formation on this surface. This innovative product is now available as a 96-well round bottom cell culture plate.
What Are Cell Culture Plates?
Cell culture plates are specialized containers designed for the cultivation and expansion of cells in laboratory settings. These ell culture dishes are available in both disposable and reusable forms, featuring shallow designs tailored to support cell growth. Dishes with pre-treated surfaces facilitate the adhesion of anchor dependent cells, whereas untreated dishes are suitable for the cultivation of suspension cells.
These dishes are offered in various sizes, with options for single- or multi-well formats, and they may have round or rectangular shapes. Typically crafted from materials like borosilicate glass or clear plastics, such as polystyrene or polycarbonate, these dishes enable distortion-free microscopic observation. Many cell culture dishes come equipped with lids that ensure consistent gas exchange while safeguarding the cultured cells from the external environment.
Material:
Polystyrene: Most commonly used material for cell culture plates due to its transparency, durability, and compatibility with various cell types.
Polycarbonate or glass: Occasionally used for specific applications, offering different properties like optical clarity or resistance to certain chemicals.
Surface Treatment:
Tissue Culture (TC) treated: Features a hydrophilic surface that promotes cell adhesion, making it suitable for adherent cell cultures.
Non treated: Has a hydrophobic surface and is often used for suspension cell cultures or applications where cell adhesion is not required.
Configuration:
Single-well or multi-well: Available in various formats, including 6, 12, 24, 48, 96, or 384 wells, catering to different experimental scales.
Round or square wells: Shape variations to suit experimental preferences or specific requirements.
Coating:
Collagen or poly-lysine coated: Some plates come pre-coated to enhance the adhesion of specific cell types that may not thrive on standard surfaces.
Lids and Sterility:
Sterile: Many cell culture plates are sold in a sterile condition to prevent contamination during cell culture experiments.
Lids or covers: Provided to create a controlled environment within each well, supporting gas exchange while protecting the cultures.
How Many Cells Should I Seed To Start A Cell Culture?
It is advised not to seed cells in a manner that would result in the average yield per well surpassing 100% confluence as cells undergo proliferation after seeding. The appropriate seeding density for your cell culture depends on factors such as the specific cell type, cell size, growth rate, and the duration of your experiment. Adjustments in seeding density may be necessary based on these variables to ensure optimal cell growth and experimental outcomes.
How Can I Choose The Right Growth Surface For My Cell Cultures?
For suspension cell culture and various biochemical assays, optically clear and non-treated polystyrene with a hydrophobic surface proves to be an excellent choice. The hydrophobic nature of the surface enhances its utility for suspension cell cultures. Conversely, a standard tissue culture (TC) surface modification transforms the polystyrene surface into a more hydrophilic one, facilitating maximum adhesion for a diverse range of cell types.
In instances where cells, particularly sensitive or primary cells, struggle to thrive on the standard TC surface, specialized cell culture dishes coated with collagen or poly-lysine can be employed. Collagen, derived from animals, and poly-lysine, a fully synthetic option, provide alternative surfaces to support the growth and adhesion of cells with specific requirements.
Do Cell Culture Plates And Dishes Have An Expiration Date?
Indeed, they do. The expiration date typically indicates the guaranteed sterility of the product. It is important to emphasize that maintaining sterility also relies on proper handling to ensure the integrity of the packaging. Certain culture vessels may contain additional substances, such as gelatin, which can degrade over time. It is advisable not to use cell culture vessels with such additives beyond their expiration date to ensure the reliability of the culture environment.
Yongkang Medical is a company that specializes in the manufacturing and distribution of high quality cell culture dish for labs or medical applications. Contact us to order 96 well flat bottom tissue culture plates.
